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live dead ghost dye violet 510  (Cytek Biosciences)


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    Structured Review

    Cytek Biosciences live dead ghost dye violet 510
    Live Dead Ghost Dye Violet 510, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/live+dead+dye/Ghost+Dye+Violet+510/pm41874176-250-10-15
    Average 96 stars, based on 545 article reviews
    live dead ghost dye violet 510 - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Excess neuropeptides in lung signal through endothelial cells to impair gas exchange.
    Article Snippet: Article Excess neuropeptides in lung signal through endothelial cells to impair gas exchange

    Article Title: CD4 + T‐Cell‐Intrinsic IL‐6 Is Critical for Th17 Differentiation and Dampened Responsiveness to CD8 + T Cell‐Mediated Suppression
    Article Snippet: Data were analyzed with FlowJo software (version 10.7.1). .. To determine the extent of cell survival and cell death, CD4 T‐cells were stained with a live/dead dye (Ghost Dye Violet 450 viability dye, Tonbo Biosciences, 13‐0863‐T100). .. Total RNAs from KO and control CD4 T‐cells (2 × 10 6 cells for each condition) were isolated using the RNeasy Mini kit according to the manufacturer's instructions (Qiagen, 74104).

    Article Title: Brainstem Dbh + neurons control allergen-induced airway hyperreactivity.
    Article Snippet: For lung lymphoid tissue, the following antibodies were used: 1:200 FITC-conjugated anti-CD45 (BioLegend, no. 103108), 1:100 APC-Cy7-conjugated anti-IL-7Ra (BioLegend, no. 135040), V450-conjugated Lineage mix (1:200 anti-CD19, TONBO, no. 50-201- 4944), 1:500 anti-CD11c (TONBO, no. 50-201-4937), 1:500 anti-F4/80 (TONBO, no. 50-201-4978), 1:100 anti-NK1.1 (BD, no. 560524), 1:100 anti-TER119 (BD, no. 560504), 1:100 anti-TCR gamma delta (Invitrogen, no. 48-5711-82), 1:100 BV510-conjugated anti-ST2 (BD, no. 745080), 1:200 PE-Cy7-conjugated anti-TCR-beta (BioLegend, no. 109222), 1:100 BV604-conjugated anti-CD4 (BioLegend, no. 100548) and 1:2,000 PerCP-Cy5.5-conjugated anti-CD90.2 (BioLegend, no. 105338). .. Cells were then stained using live/dead dye (1:1,000, Ghost Dye Red 780 (TONBO, no. 13-0865-T100 for myeloid tissue, 1:500 and Ghost Dye Violet 450, TONBO, no. 13-0863-T100)) before fixing using BD Stabilizing Fixative and transfer to fluorescent activated cell sorting tubes. .. Goblet cells were stained using a PAS staining kit (Sigma).

    Article Title: Tumor-intrinsic PRC2 inactivation drives a context-dependent immune-desert microenvironment and is sensitized by immunogenic viruses
    Article Snippet: First, cells were stained with an Fc-blocking antibody for 15 minutes and then with an Fc-blocking antibody plus iTAg H-2Kb OVA Tetramer-SIINFEKL-APC (MBL International, catalog TB-5001-2) in FACS buffer for 1 hour at room temperature and protected from light. .. Next, live/dead dye (TONBO, catalog 13-0863- T100) and anti–mouse CD8a-FITC (TONBO, catalog 35-1886-U100) were added to the staining system for 30 minutes at 4°C. ..

    Article Title: The CD155/TIGIT axis promotes and maintains immune evasion in neoantigen-expressing pancreatic cancer.
    Article Snippet: In brief Freed-Pastor et al. identify the CD155/ TIGIT axis as a key driver of immune evasion in pancreas cancer.. Neoepitope prediction reveals a subset of human pancreas cancer patients with predicted high-affinity neoepitopes and functional interrogation using preclinical models identifies a combination immunotherapy approach (TIGIT/PD-1 co-blockade plus CD40 agonism) capable of eliciting profound anti-tumor responses.

    Blocking Assay:

    Article Title: Excess neuropeptides in lung signal through endothelial cells to impair gas exchange.
    Article Snippet: Article Excess neuropeptides in lung signal through endothelial cells to impair gas exchange

    Incubation:

    Article Title: TNFR2 signaling in oligodendrocyte precursor cells suppresses their immune-inflammatory function and detrimental microglia activation in CNS demyelinating disease.
    Article Snippet: Multiple Sclerosis (MS) is a chronic degenerative disease of the central nervous system (CNS) characterized by inflammation, demyelination, and progressive neurodegeneration.. These processes, combined with the failure of reparative remyelination initiated by oligodendrocyte precursor cells (OPCs), lead to irreversible neurological impairment.. The cytokine tumor necrosis factor (TNF) has been implicated in CNS repair via activation of its cognate receptor TNFR2 in glia.

    FACS:

    Article Title: Brainstem Dbh + neurons control allergen-induced airway hyperreactivity.
    Article Snippet: For lung lymphoid tissue, the following antibodies were used: 1:200 FITC-conjugated anti-CD45 (BioLegend, no. 103108), 1:100 APC-Cy7-conjugated anti-IL-7Ra (BioLegend, no. 135040), V450-conjugated Lineage mix (1:200 anti-CD19, TONBO, no. 50-201- 4944), 1:500 anti-CD11c (TONBO, no. 50-201-4937), 1:500 anti-F4/80 (TONBO, no. 50-201-4978), 1:100 anti-NK1.1 (BD, no. 560524), 1:100 anti-TER119 (BD, no. 560504), 1:100 anti-TCR gamma delta (Invitrogen, no. 48-5711-82), 1:100 BV510-conjugated anti-ST2 (BD, no. 745080), 1:200 PE-Cy7-conjugated anti-TCR-beta (BioLegend, no. 109222), 1:100 BV604-conjugated anti-CD4 (BioLegend, no. 100548) and 1:2,000 PerCP-Cy5.5-conjugated anti-CD90.2 (BioLegend, no. 105338). .. Cells were then stained using live/dead dye (1:1,000, Ghost Dye Red 780 (TONBO, no. 13-0865-T100 for myeloid tissue, 1:500 and Ghost Dye Violet 450, TONBO, no. 13-0863-T100)) before fixing using BD Stabilizing Fixative and transfer to fluorescent activated cell sorting tubes. .. Goblet cells were stained using a PAS staining kit (Sigma).

    Labeling:

    Article Title: Allergen induces pulmonary neuroendocrine cell hyperplasia in a model of asthma
    Article Snippet: For lung lymphoid cells, the following antibodies were used: 1:200 FITC-conjugated anti-CD45 (BioLegend, no. 103108), 1:100 APC-Cy7-conjugated anti–IL-7Ra (BioLegend, no. 135040), 1:200 V450-conjugated lineage mix (anti-CD19 [TONBO, no. 50-201-4944], anti-CD11c [TONBO, no. 50-201-4937], anti-F4/80 [TONBO, no. 50-201-4978], anti-NK1.1 [BD Biosciences, no. 560524], anti-TER119 [BD Biosciences, no. 560504], anti-TCR γδ [Invitrogen, no. 48-5711-82]), 1:100 BV510-conjugated anti-ST2 (BD Biosciences, no. 745080), 1:200 PE-Cy7-conjugated anti–TCR-β (BioLegend, no. 109222), 1:100 BV604-conjugated anti-CD4 (BioLegend, no. 100548), anti–cleaved caspase-3 (Cell Signaling Technology, Asp175, no. 9661), anti-Ki67 (Cell Signaling Technology D3B5, no. 9129), and 1:2,000 PerCP-Cy5.5-conjugated anti-CD90.2 (BioLegend, no. 105338). .. Cells were labeled with live/dead dye (Ghost Dye Red 780, TONBO, no. 13-0865-T100 or Ghost Dye Violet 450, TONBO, no. 13-0863-T100), then fixed using BD Biosciences Stabilizing Fixative. .. Samples were analyzed using a BD Biosciences FACSCanto RUO – ORANGE flow cytometer (405, 488, and 640 nm lasers) at the VA San Diego Health Care System Flow Cytometry Core.

    other:

    Article Title: Allergen induces pulmonary neuroendocrine cell hyperplasia in a model of asthma.
    Article Snippet: The GentleMACS “mouse lung 1-2” program (Miltenyi Biotec) was used for initial dissociation.



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